Skip to main content
. 2022 Aug 26;16(10):15792–15804. doi: 10.1021/acsnano.2c04232

Figure 3.

Figure 3

Representative super-resolution microscopy images of PA14 cells after 4 h incubation with (A) pMPCylated and (B) PEGylated-liposomes at 37 °C. Inset shows a zoomed-in detail of bacteria cells with liposomes adhering to the membrane. Images are presented as overlays of bacteria membrane stained with FM1–43 dye (green) with liposomes labeled with DiR dye (red) visualized using stochastic optical reconstruction microscopy (STORM). Scale bar, 1 μm; insert scale bar 1 μm. (C) Quantification of bacteria cells containing liposomal fluorescence from STORM images. Each fluorescent locus on the cells’ membrane with a minimum size of 180 nm was counted as one individual cell-attached liposomal unit. Differences between groups shown in the box plot were tested with a one-way ANOVA. Boxes represent the 25–75 percentiles of the sample distribution, with black vertical lines representing 1.5 × IQR (interquartile range). Black horizontal line represents the median.