Kinetics of loss of accessory cell function for alcohol-treated and untreated 43HIV cells. 43HIV cells were pretreated with ethanol at 0 mM (A), 25 mM (B), 75 mM (C), and 150 mM (D) for 16 h and then pulsed with various concentrations of PHA (1 to 0.01 μg/ml), ConA (1 to 0.01 μg/ml), PWM (1 to 0.01 μg/ml), and MAb 446 (1 μg/ml) after 72 h TT (4 μg/ml)-induced T-cell proliferation was measured after 5 days. T-cell proliferation after mitogen, antigen, and anti-CD3 stimulation was determined by thymidine incorporation.