TABLE 1.
Effects of ethanol, acetaldehyde, and acetate on accessory cell function in clone 43 cellsa
| Treatment (mM) | Fold increase after stimulation with b:
|
||||
|---|---|---|---|---|---|
| PHA | ConA | PWM | MAb 446 | TT | |
| None | 135 ± 64 | 70 ± 34 | 28 ± 14 | 20 ± 9 | 6 ± 3 |
| Ethanol | |||||
| 25 | 103 ± 34 | 43 ± 21 | 18 ± 9 | 15 ± 7 | 4 ± 2 |
| 75 | 40 ± 17 | 14 ± 7 | 13 ± 4 | 11 ± 3 | 3 ± 1.5 |
| 150 | 14 ± 8 | 9 ± 5 | 7 ± 3 | 5 ± 3 | 1 ± 0.5 |
| Acetaldehyde | |||||
| 25 | 99 ± 37 | 45 ± 33 | 17 ± 1 | 14 ± 7 | 4.5 ± 1.7 |
| 75 | 39 ± 16 | 15 ± 8 | 14 ± 5 | 13 ± 7 | 3.1 ± 1.6 |
| 150 | 13 ± 7 | 8 ± 4 | 10 ± 5 | 10 ± 4 | 1.8 ± 0.9 |
| Acetate | |||||
| 25 | 101 ± 38 | 45 ± 23 | 20 ± 9 | 16 ± 8 | 4.5 ± 3.1 |
| 75 | 42 ± 20 | 15 ± 8 | 12 ± 5 | 10 ± 4 | 3.1 ± 1.5 |
| 150 | 15 ± 7 | 10 ± 6 | 7.5 ± 4 | 6 ± 2 | 1.1 ± 0.9 |
Some clone 43 cells (104) were maintained in culture, while others were treated with various concentrations (25, 75, and 150 mM) of ethanol, acetaldehyde, and acetate for 16 h, washed with PBS, cocultured with 105 monocyte-depleted PBMCs, and stimulated with PHA (1 μg/ml), ConA (1 μg/ml), PWM (1 μg/ml), and MAb 446 (anti-CD3 [5 μg/ml]) for 72 h. TT (4 μg/ml)-stimulated cultures were harvested after 5 days. [3H]thymidine (1 μCi) was added for the last 16 h, the cells were harvested, and the incorporated counts per minute were analyzed.
Data are the means ± standard errors from the pooled data of three separate experiments and are expressed as fold increases (stimulation index) in accessory cell function over levels in unstimulated cultures.