TABLE 2.
Effects of ethanol, acetaldehyde, and acetate on accessory cell function in monocytesa
| Treatment (mM) | Fold increase after stimulation withb:
|
||||
|---|---|---|---|---|---|
| PHA | ConA | PWM | MAb 446 | TT | |
| None | 265 ± 70 | 110 ± 30 | 57 ± 18 | 27 ± 9 | 7 ± 3 |
| Ethanol | |||||
| 25 | 151 ± 50 | 67 ± 20 | 35 ± 10 | 19 ± 8 | 4.5 ± 1.8 |
| 75 | 81 ± 24 | 53 ± 18 | 21 ± 7 | 12 ± 5 | 2.5 ± 1 |
| 150 | 27 ± 9 | 19 ± 9 | 10 ± 4 | 7 ± 2 | 1 ± 0.9 |
| Acetaldehyde | |||||
| 25 | 45 ± 51 | 71 ± 22 | 38 ± 14 | 21 ± 10 | 5 ± 2.5 |
| 75 | 78 ± 20 | 52 ± 23 | 19 ± 7 | 13 ± 8 | 3 ± 1 |
| 150 | 30 ± 11 | 21 ± 7 | 11 ± 5 | 8 ± 3 | 1 ± 0.7 |
| Acetate | |||||
| 25 | 148 ± 45 | 71 ± 21 | 31 ± 13 | 23 ± 10 | 5 ± 2 |
| 75 | 79 ± 20 | 57 ± 19 | 2 ± 10 | 13 ± 4 | 3 ± 1.1 |
| 150 | 29 ± 10 | 17 ± 9 | 12 ± 5 | 8 ± 3 | 1.5 ± 0.7 |
a
Some cells (104) were maintained in culture, while others were treated with various concentrations of ethanol, acetaldehyde, and acetate as described for Table 1.
b
Data are the means ± standard errors from the pooled data of three separate experiments and are represented as fold increases (stimulation index) in accessory cell function over levels in unstimulated cultures.