Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 17;10:1035543. doi: 10.3389/fbioe.2022.1035543

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Rittiner, Cumaran, Malhotra and Kantor.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

CRISPRa (activation) and CRISPRi/r (interference/repression). The synthetic activators (upper panel) comprise of dead/inactive Cas9 (dCas9) fused with transcriptional activators, e.g. VP64, p65, and RTA encoded by Epstein-Barr virus (EBV). Transcriptional repression (lower panel) schematically represented by dCas protein fused with DNA methyltransferase, here, DNMT3A and DNMT3L. The methylated DNA could be then repressed via the cascade of DNA-methylation mediated events.