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. 2022 Oct 31;14:124. doi: 10.1186/s13073-022-01131-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Mycophenolate mofetil (MMF) treatment enhances ganciclovir (GCV)-associated mutagenesis. Fraction of CA > AA mutations in the H414 cell line (A), H023 organoid (B), and the HCT116 cell line (C) treated with vehicle (DMSO), MMF, ACV, ACV + MMF, GCV, or GCV + MMF. The concentration of MMF is 1 µM in all treatments. For H414 and HCT116 cells, the concentration of ACV and GCV is 100 µM. For H023, the concentration of GCV is 20 µM and 40 µM (2 ×). D GCV^sig contribution score computed using sigfit for the mutational spectra of the treated cell lines and organoids in A–C. E Doubling time of H414 cells under different treatment conditions. GCV concentration was constant at 100 µM. The line indicates the mean of replicates. F Liquid chromatograph-tandem mass spectrometry-based quantification of GCV incorporation into genomic DNA under increasing MMF dosage in 100 µM GCV-treated H414 cells. The line indicates the mean of replicates