FIG. 2.

Concentration of oxLDL required for maximal absorption of free anti-oxLDL antibodies. Each point in the figure corresponds to the difference in OD readings at 414 nm between unabsorbed and absorbed aliquots of our reference purified antibody and serum D (see Table 1). Identical dilutions of the antibody preparations (1/2 for isolated antibody, 1/20 for serum) were absorbed with increasing concentrations of oxLDL, as shown in the figure. In the unabsorbed aliquots, oxLDL was replaced by PBS. Each value corresponds to the mean duplicate values ± 1 standard deviation.