Table 7.

Validation Parameters of PCA, PLS-DA and OPLS-DA Models for Mice Feces, Livers, Serum and Urine

Samples		NC vs UC				SYD vs UC				NC vs UC vs SYD
		PLS-DA		OPLS-DA		PLS-DA		OPLS-DA		PCA
		ESI (+)	ESI (-)	ESI (+)	ESI (-)	ESI (+)	ESI (-)	ESI (+)	ESI (-)	ESI (+)	ESI (-)
Feces	Significant components	3	3	3	4	2	2	2	2	6	6
	R2X	0.605	0.615	0.605	0.650	0.480	0.424	0.480	0.424	0.735	0.717
	Q2	0.935	0.900	0.926	0.898	0.811	0.709	0.822	0.723	0.546	0.496
	p	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	–	–
Livers	Significant components	3	4	2	8	3	2	5	3	5	6
	R2X	0.652	0.690	0.596	0.843	0.525	0.348	0.690	0.512	0.758	0.770
	Q2	0.972	0.862	0.961	0.930	0.747	0.354	0.846	0.477	0.466	0.533
	p	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	–	–
Serum	Significant components	3	4	3	2	2	3	3	3	3	2
	R2X	0.729	0.915	0.729	0.824	0.480	0.562	0.626	0.562	0.733	0.753
	Q2	0.912	0.909	0.904	0.677	0.132	0.395	0.264	0.412	0.413	0.273
	p	<0.05	<0.05	<0.05	<0.05	>0.05	<0.05	>0.05	<0.05	–	–
Urine	Significant components	2	2	5	2	3	2	2	3	3	5
	R2X	0.493	0.521	0.747	0.521	0.655	0.535	0.585	0.618	0.622	0.759
	Q2	0.908	0.900	0.943	0.907	0.933	0.882	0.900	0.891	0.402	0.445
	p	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	–	–

Notes: R²X represents the goodness of fit of models; Q² represents the predictability of models; p values were calculated by CV-ANOVA; PLS-DA and OPLS-DA model is valid when p < 0.05. NC vs UC: compare Normal Control group with Model group; SYD vs UC: compare SYD group with Model group; NC vs UC vs SYD: compare Normal Control, SYD group with Model group.