Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 6;18(5):2079346. doi: 10.1080/21645515.2022.2079346

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

PMC Copyright notice

Figure 1. — Interaction and binding of RBD-J and CpG to aluminum salt adjuvants (AH and AP). (a) Reducing SDS-PAGE to determine binding of RBD-J to AH and AP in the absence (highlighted in the red box, Lanes #6–9) and presence (highlighted in the blue box, Lanes #10–13) of CpG 1018. U= Unbound RBD-J, B = Bound RBD-J. Lanes #1–5 are increasing amounts of unadjuvanted in-solution RBD-J to generate standard curve for RBD-J quantitation. Lane #14 is a molecular weight standard. (b) Representative spectra from densitometric analysis of gel using ImageJ software along with the standard curve generated to quantify amount of unbound and bound protein (c) Representative UV-Visible spectroscopy spectra for CpG in unbound (U) and bound fractions (B) after adsorption to AH and AP, and (d) summary table with compiled results for percentage of RBD-J and CpG bound to AH and AP. *LOQ = 15%.