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. 2022 Jun 6;18(5):2079346. doi: 10.1080/21645515.2022.2079346

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 5. — Conformational stability of RBD-J in formulations F1-F7 as measured by DSC. (a) Representative DSC thermograms of RBD-J in each formulation. DSC thermogram of unadjuvanted in-solution RBD-J (black) is plotted alongside each adjuvanted formulation for comparison. Thermal unfolding temperature (T_m) values are shown as the mean of n = 2 measurements with range = ± 0.1–0.6°C. Effect of adjuvants on (b) ΔT_onset and ΔT_m values and (c) Δapparent enthalpy (∆∆H’) values of RBD-J in F1-F6 as determined by subtracting the results for each formulation from respective values of the unadjuvanted in-solution RBD-J control. Bars indicate mean of n = 2 measurements and the errors bars represent the data range.