Figure 6.
In vivo mouse immunogenicity of AH+CpG-adjuvanted RBD-J formulation stored at 4°C and 25°C for two months. (a) Mice (n = 6) were immunized by subcutaneous route with two 5 mcg doses (days 0 and 21) of AH+CpG-adjuvanted RBD-J stored at 4 and 25°C for 2 months. Serum was collected on days 21, 35, and 65. (b) Sera anti-RBD-J IgG titer as determined by ELISA and (c) Individual NT50 values as determined using a best-fit nonlinear regression on serum dilution curves on day 21, 35, and 65. The dashed lines represent group mean, and the errors bars indicate standard deviation. Statistical significance between time-points in each group was determined by Kruskal-Wallis test and post hoc Dunn’s multiple comparisons test (**p ≤ 0.01). Illustration in (a) was created with Biorender.com.