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. 2022 Oct 27;15:3285–3301. doi: 10.2147/DMSO.S380053

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© 2022 Tong et al.

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FST expression increased in the FFA-treated human liver (LO2) cells. (A) CCK-8 assay results showed the viability of LO2 cells treated with different concentrations of FFA. (B and C) Oil red O staining and analysis result of LO2 cells with the control (CON) and 1 mM FFA treatment (400×, scale bar: 50 µm). (D and E) The protein expression and analysis results of FST, ACC1, FASN, SREBP1, and ChREBP in LO2 cells with the control and 1 mM FFA treatment. β-actin was used as a loading control.

Notes: NC group was LO2 cells without FFA treatment. control (CON) group was LO2 cells treated with BSA. *P< 0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001 vs NC group or CON group.

Abbreviations: FST, follistatin; FFA, free fatty acid; BSA, bovine serum albumin (fatty acid free); SREBP1, sterol regulatory element-binding protein1; ChREBP, carbohydrate-responsive element-binding protein; ACC1, acetyl-CoA carboxylase1; FASN, fatty acid synthase.