Numbers of differentially methylated genes (DMGs) and their enriched gene pathways after treatment of 17β-oestradiol (E2). (a) Comparison of DMGs after E2 treatment in females and males at 3, 9, 12, 16, and 110 days post fertilization (dpf). B-E KEGG (Kyoto Encyclopaedia of Genes and Genomes) pathway enrichment analysis of hypermethylated methylated genes (DMGs) in females (b), hypomethylated DMGs in females (c), hypermethylated DMGs in males (d), and hypomethylated DMGs in males (e) at 3 and 16 dpf after E2 treatment.