Figure 4.

Desmosome architectural changes correspond to E-cadherin exclusion in individual junctions. (a) Representative two-color dSTORM images of desmosomes in MDCK cells over time (magenta: desmoplakin rod/C-terminal junction and cyan: E-cadherin). Quantification of the desmoplakin plaque-to-plaque distance (b) and plaque length (c) according to whether E-cadherin was present (cyan) or not (magenta). (3 h n = 25, 6 h n = 25, 8 h n = 25, 12 h n = 25, and 36 h n = 25) Scale bar = 0.5 μm. * P ≤ .05, ** P ≤ .01, and *** P ≤ .001 by ANOVA with post hoc Tukey’s test.