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. Author manuscript; available in PMC: 2022 Oct 31.
Published in final edited form as: Biotechnol Bioeng. 2020 Sep 24;118(1):186–198. doi: 10.1002/bit.27561

FIGURE 1.

FIGURE 1

Knockout of the CASP8AP2 gene. (a) Agarose gel electrophoresis of polymerase chain reaction (PCR) amplified CASP8AP2 locus in the parental and the 892–7 cell lines, and EMBOSS WATER sequence alignment of both alleles with the parental sequence. Guide RNA (gRNA) target sequence is highlighted. GAPDH-normalized qPCR analysis of CASP8AP2 messenger RNA (mRNA) abundance in parental and CASP8AP2 knockout clone, 892–7, using primers targeting the gRNA locus for amplification. (b) Caspase 8 activity in cell lysates of control (uninduced) and apoptosis-induced (induced) 892–7 and parental cells (**p < .001)