Skip to main content
. Author manuscript; available in PMC: 2022 Oct 31.
Published in final edited form as: Biotechnol Bioeng. 2020 Sep 24;118(1):186–198. doi: 10.1002/bit.27561

FIGURE 2.

FIGURE 2

Luciferase and secreted alkaline phosphatase (SEAP) production from the parental cell line and the CASP8AP2 knockout clone, 892–7. (a) Luciferase enzymatic activity (***p < .0001). (b) GAPDH-normalized luciferase messenger RNA (mRNA) abundance determined using real-time quantitative polymerase chain reaction (** p < .001). (c) Luciferase expression by Western blot analysis. (d) Alkaline phosphatase activity in the cell culture supernatant of cells transfected with a plasmid encoding the SEAP gene (*p < .05). Results represent the average of at least three biological replicates, and error bars represent the standard deviation