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. Author manuscript; available in PMC: 2022 Oct 31.
Published in final edited form as: Biotechnol Bioeng. 2020 Sep 24;118(1):186–198. doi: 10.1002/bit.27561

FIGURE 4.

FIGURE 4

The effect of CASP8AP2 knockout on cell cycle progression by FACS-based, propidium iodide cell cycle analysis. (a) Knockout clone. (b) Parental cells. (c) Percentages of cells in the G1, S, or G2/M in asynchronous cultures calculated using BD FACS DIVA software. Results represent the average of three analyses, and error bars represent the standard deviation (*p < .05). (d) Parental and 892–7 cells were treated with thymidine to synchronize the cell cycle. After release from the thymidine block, the cells were harvested at the indicated time points and cell cycle progression was analyzed using a cytometer