Skip to main content
. 2022 Nov 1;132(21):e151363. doi: 10.1172/JCI151363

Figure 4. Glandular regeneration after HDT and upon chief cell loss is mediated through YAP.

Figure 4

(A) Immunofluorescence costaining for nonphosphorylated YAP (red) and E-cadherin (white) in Rspo3-WT mice treated with HDT for 2 days and sacrificed on day 1, 3, or 7 after treatment and nontreated control. (B) qPCR data showing expression of YAP target genes Ctgf, Igfbp3, and Cyr61 and the transcription factor Tead4 in the corpus of Rspo3-WT mice treated with HDT for 2 days and sacrificed on day 1 (n = 6) versus nontreated controls (n = 4). (C) Immunofluorescence staining for nonphosphorylated YAP (red) and E-cadherin (white) in Rspo3-WT and Rspo3-KO mice treated with HDT for 2 days and sacrificed on day 1 after treatment. (D) Quantification of YAP+ nuclei per image excluding parietal cells in Rspo3-WT and Rspo3-KO mice treated with HDT for 2 days and sacrificed on day 1 or day 3 after treatment and nontreated control. (E) Immunofluorescence costaining for nonphosphorylated YAP (red) and E-cadherin (white) in LGR5DTR mice treated with DT on 3 consecutive days and sacrificed on day 1 after treatment and nontreated littermates. (F) qPCR data showing expression of YAP target genes Ctgf, Igfbp3, and Cyr61 and the transcription factor Tead4 in the corpus of LGR5DTR mice (n = 3) treated with DT on 3 consecutive days and sacrificed on day 1 after treatment versus nontreated littermate controls (n = 3). Scale bars: 50 μm. Unpaired parametric t test (B and F); 1-way ANOVA with Tukey’s multiple-comparison test (D).