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. 2022 Nov 1;132(21):e151363. doi: 10.1172/JCI151363

Figure 6. RSPO3 causes glandular proliferation upon H. pylori infection.

Figure 6

(A) H&E staining of 2-month–infected Rspo3-KI mice. (B) Representative immunofluorescence images of costaining for GSII (red) and GIF (green) of sections from noninfected and 2-month–infected Rspo3-WT and Rspo3-KI mice. (C) Representative immunofluorescence images of Ki67 staining (white) and MUC5AC (red) of sections from noninfected and 2-month infected Rspo3-WT and Rspo3-KI mice. (D) Quantification of Ki67+ cells per gland of infected Rspo3-WT (n = 4 mice) and Rspo3-KI mice (n = 5) and noninfected littermate controls (n = 3–4). (E) Quantification of proliferating cells per gland base of infected Rspo3-WT (n = 7) and Rspo3-KI mice (n = 5) and noninfected littermate controls (n = 4–5). (F and H) GSEA of microarray data comparing the expression profile of the corpus tissue from 2-month–infected Rspo3-KI mice and infected littermate controls treated with tamoxifen 2 months before euthanasia with a published data set for (F) SPEM (28). (H) Early gastric cancer (GC) signature (29). (G and I) GSEA of microarray data comparing the expression profile of the corpus tissue from noninfected Rspo3-KI mice and noninfected littermate controls treated with tamoxifen 14 days before euthanasia with a published data set for (G) SPEM (28). (I) Early gastric cancer signature (29). ES, enrichment score; NES, normalized enrichment score. For GSEA, n = 2 mice per group. Scale bars: 100 μm. One-way ANOVA with Tukey’s multiple-comparison test.