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22RV1 cell number is plotted against qPCR Cq (n = 47 for each gene). Four genes, SYP, CHGA, AR-V7, and AR-V9, were selected for their variable expression in CTCs from patients with metastatic prostate cancer. qPCR is able to reliably detect RNA as low as an equivalent of 0.4 cells; above this, the relationship between Cq and input amount appears to be linear.
