Figure 4. Myocardial accumulation of CD69⁺ Tregs and Il-17⁺ γδT cells after LAD ligation.

(A) Leukocyte cell numbers per milligram of heart tissue in the myocardium 2 days after infarction. (B) Quantification of the number of Tregs (CD4⁺Foxp3⁺) and CD4⁺Foxp3⁺CD69⁺ cells per milligram of heart tissue and CD69 mean fluorescence expression on Tregs in the heart. Representative density plots showing gating on CD45⁺CD11b^–CD4⁺ cells. (C) Representative density plots gated on CD45⁺CD11b^–CD3⁺ cells and numbers of γδT cells and Il-17⁺ γδT cells per milligram of tissue. (D) Quantification of total cell numbers per milligram of CD11b⁺ myeloid cells, CD11b⁺Gr1^hi cells, and CD11b⁺F4/80^loLy6C^hi cells in the heart. Heart cell–infiltrating populations were evaluated 2 days after infarction (n = 6–11 animals per group). Data are representative of 4 independent experiments and indicate the mean ± SEM. Statistical significance was analyzed by 1-way ANOVA with Tukey’s post hoc test. P values for significant differences are shown.