Fig 6. K2 treatment renders K. pneumoniae more susceptible to intracellular killing.

A. The effect of K2 on intracellular killing was assessed by adding it at different steps of the experimental process. K2 was added either at every step (bacterial overnight culture, D. discoideum overnight culture and during the ingestion and killing of bacteria; blue), or only in the overnight bacterial culture (red), and compared to a treatment with DMSO (mean ± SEM; *: p<0.05; Kruskal-Wallis test, Dunn’s test. N = 5 independent experiments). B. The corresponding survival curves with K2 added at every step (blue) or only during the bacterial preculture (red) are shown.