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. 2022 Sep 27;11:e81088. doi: 10.7554/eLife.81088

Figure 3. Ovary folding is concomitant with specification of the medullary and cortical compartments of the ovary.

Figure 3.

(A, C, E) Optical sections from confocal Z-stacks of whole ovary/mesonephros complexes from Lgr5-Gfp mice at E14.5, E15.5, and P0 immunostained for GFP (red) and FOXL2 (cyan), and counterstained with Hoechst nuclear dye (grayscale). Images in the top row were taken from the dorsal side and the bottom row from the ventral (E14.5) or lateral (E17.5; P0). Yellow asterisks indicate the location of the infundibulum of the presumptive oviduct for reference. (B, D, F) 3D models generated by isosurface segmentation of lightsheet images taken of whole ovaries from Lgr5-Gfp mice at E14.5, E17.5, and P0 immunostained for FOXL2 (cyan) and GFP (red). Top panels represent the dorsal view, middle panels represent the medial view, and small bottom panels illustrate ventral and lateral views of the same ovary. Yellow dashed arrows in (D) indicate the wrapping of the ovarian surface epithelium (OSE) from the ventral to the dorsal side of the ovarian domain. White arrows point to the OSE. Cx, cortex; H, hilum; MD, Müllerian duct; Md, medulla; Mn, mesonephros. Compasses on the bottom left of each panel indicate the orientation of the ovary for the entire row: A, anterior; D, dorsal; L, lateral; M, medial; P, posterior; V, ventral. Scale bars, 100 μm.