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. 2022 Sep 27;11:e81088. doi: 10.7554/eLife.81088

Figure 4. The expansion and relocation of the Müllerian duct leave the ovary fully encapsulated.

Figure 4.

(A) 3D model generated by isosurface segmentation of lightsheet images taken of whole ovaries at E14.5, E17.5, and P0 immunostained for FOXL2 (cyan) and PAX8 (red). A, anterior pole; P, posterior pole. (B) Maximum intensity projections from confocal Z-stacks of whole ovary/mesonephros complexes at E14.5 (B, left), E17.5 (B, middle), and P0 (B, right) immunostained for PAX8 (red) and ENDOMUCIN (cyan), and counterstained with Hoechst nuclear dye (grayscale). Images in the top row were taken from the dorsal side and the bottom row from the lateral side. Yellow asterisks indicate the location of the infundibulum of the presumptive oviduct for reference. (C) 3D models generated by isosurface segmentation of lightsheet images taken of whole ovaries at E14.5 (C, left), E17.5 (C, middle), and P0 (C, right) immunostained for FOXL2 (cyan) and PAX8 (red), and counterstained with Hoechst nuclear dye (grayscale). The isosurfaces generated with the gray channel allow for visualization of the entire tissue, including the ovarian capsule. White arrowheads point to the Hoechst-based surface of the ovary, which extends beyond the FOXL2+domain, and should not be confused with the ovarian capsule. Top panels represent the ventral view, middle panels represent the lateral view, and small bottom panels illustrate dorsal and medial views of the same ovary. Green arrows indicate the change in position of the infundibulum relative to the longitudinal plane of the ovary. Green asterisks indicate the approximate center of the ovary. White double arrows indicate the extension of mesonephric tissue around the ovary in the anterior (left arrows) and posterior regions (right arrows). Yellow asterisks indicate the location of the infundibulum of the presumptive oviduct for reference. H, hilum; MD, Müllerian duct; Mn, mesonephros; OA, ovarian artery; OC, ovarian capsule; Ov, ovary; Ovi, oviduct; Ut, uterus. Compasses on the bottom left of each panel indicate the orientation of the ovary for the entire row: A, anterior; D, dorsal; L, lateral; M, medial; P, posterior; V, ventral. Scale bars, 100 μm.