| Strain, strain background (Mus musculus) |
Crl:CD1(ICR) |
Charles River |
Strain code: 022 |
|
| Strain, strain background (M. musculus) |
C57BL/6J |
Jackson Laboratory |
Stock #:000664 |
|
| Genetic reagent (M. musculus) |
B6.129P2-Lgr5tm1(cre/ERT2)Cle/J |
Jackson Laboratory |
Stock #:008875 |
|
| Genetic reagent (M. musculus) |
Tg(Nr5a1/EGFP)1Klp |
PMID:12351700
|
MGI:5493455 |
|
| Genetic reagent (M. musculus) |
Tg(Wnt7a-EGFP/cre)#Bhr/Mmjax |
PMID:20974921
|
MMRRC Strain #036637-JAX |
|
| Genetic reagent (M. musculus) |
Pax8tm1Rdl; Pax2tm1a(KOMP)Wtsi |
PMID:32381599
|
|
|
| Antibody |
AMH (goat polyclonal) |
Santa Cruz Biotechnology |
#sc-6886 |
1:500 |
| Antibody |
Smooth muscle alpha action (aSMA) (Cy3-conjugated mouse monoclonal) |
Sigma-Aldrich |
C6198 |
1:1000 |
| Antibody |
Smooth muscle alpha action (aSMA) (FITC-conjugated mouse monoclonal) |
Sigma-Aldrich |
F3777 |
1:500 |
| Antibody |
E-Cadherin (rat monoclonal) |
Zymed (Thermo Fisher Scientific) |
13-1900 |
1:500 |
| Antibody |
Endomucin (rat monoclonal) |
Santa Cruz Biotechnology |
sc-65495 |
1:500 |
| Antibody |
FOXL2 (goat polyclonal) |
Novus Biologicals |
NB-100-1277 |
1:250 |
| Antibody |
GATA4 (goat polyclonal) |
Santa Cruz Biotechnology |
sc-1237 |
1:250 |
| Antibody |
GFP (chicken polyclonal) |
Abcam |
ab13970 |
1:1000 |
| Antibody |
HuC/D (human polyclonal) |
Gift from V. Lennon (Mayo Clinic) |
|
1:10,000 |
| Antibody |
KRT8 (rat monoclonal) |
DSHB |
TROMA-I |
1:250 |
| Antibody |
PAX8 (rabbit polyclonal) This antibody has cross-reactivities with other PAX family members
|
Proteintech; Moretti et al., 2012
|
A10336-1-AP |
1:500 |
| Antibody |
RUNX1 (rabbit monoclonal) |
Abcam |
ab92336 |
1:500 |
| Antibody |
SOX9 (rabbit polyclonal) |
Millipore |
AB5535 |
1:1000 |
| Antibody |
TNC (rabbit polyclonal) |
Gift from H. Erickson (Duke University) |
|
1:250 |
| Antibody |
TUJ1 (rabbit polyclonal) |
Abcam |
ab18207 |
1:1000 |
| Antibody |
AF647 anti-Rabbit (donkey polyclonal) |
Jackson ImmunoResearch |
711-605-152 |
1:500 |
| Antibody |
AF488 anti-Chicken (donkey polyclonal) |
Jackson ImmunoResearch |
703-545-155 |
1:500 |
| Antibody |
AF488 anti-Human (donkey polyclonal) |
Jackson ImmunoResearch |
709-545-149 |
1:500 |
| Antibody |
AF488 anti-Rat (donkey polyclonal) |
Life Technologies |
A-21208 |
1:500 |
| Antibody |
Cy3 anti-Goat (donkey polyclonal) |
Jackson ImmunoResearch |
705-165-147 |
1:500 |
| Antibody |
Cy3 anti-Chicken (donkey polyclonal) |
Jackson ImmunoResearch |
705-165-155 |
1:500 |
| Antibody |
Cy3 anti-Rat (donkey polyclonal) |
Jackson ImmunoResearch |
712-165-150 |
1:500 |
| Chemical compound, drug |
CF-647-hydrazide probe (to label Elastin) |
MilliporeSigma |
SCJ4600046 |
1:500 |
| Chemical compound, drug |
Dichloromethane |
MilliporeSigma |
270997-1L |
|
| Chemical compound, drug |
Benzyl Ether |
MilliporeSigma |
108014-1KG |
|
| Chemical compound, drug |
Heparin |
MilliporeSigma |
H3393-50KU |
|
| Chemical compound, drug |
Quadrol = N,N,N′,N′-Tetrakis(2-Hydroxypropyl)ethylenediamine |
MilliporeSigma |
122262 |
|
| Software, algorithm |
Zen Black Edition |
Carl Zeiss |
|
|
| Software, algorithm |
Imaris v9.6 |
Bitplane |
|
|
| Software, algorithm |
Adobe Creative Cloud |
Adobe |
|
Photoshop, Illustrator, Premier Pro |
