Extended Data Fig. 4. Microtubule acetylation fine tunes cell stiffness to control cell and in turn cluster migration.
(a–d) In-silico results for the predicted behaviour of controls, hyperacetylated and hypoacetylated cells and clusters as simulated on stiff substrates. (a–c) Cell tracks depicting individual cell trajectories (note the differences in the x and y-axes scales when comparing, highlighted in red); (d) Rg2 in silico calculations showing cell migration under the indicated conditions, line represents mean, and shadow SD. (e) Schematic depicts our dispersion assay (detailed in Methods). (f–i) ex vivo results for the behaviour of control, hyperacetylated and hypoacetylated neural crest cells and clusters migrating in a stiff substrate, conditions as indicated. (f–h) Cell tracks depicting individual cell trajectories; (i) Rg2 ex vivo calculations showing cell migration under the indicated conditions, line represents mean, and shadow SD. Results displayed here are representative examples from at least 3 independent experiments or simulations. f–i are related to Supplementary Movie 1.