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. 2022 Jun 21;18(8):2952–2965. doi: 10.1007/s12015-022-10393-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Protein expression changes and epithelial-to-mesenchymal (EMT) markers in neural crest differentiation. (a) Multipotency of generated NCR cells (P5 to P9) was confirmed by its ability to differentiate towards peripheral neuronal lineage (co-expressing BRN3A and MAP2), as well as into (b) adipocyte (stained positive for oil red O), (c) osteocyte (stained positive for alizarin red) and (d) chondrocyte phenotypes (stained positive for alcian blue). (e) Immunoblot analysis comparing the expression of neural stemness (SOX2, ZEB1), EMT (TWIST1, SNAI1, SLUG/SNAI2, HNK1) and NCR and MSC-related proteins (ITGB1/CD29; TFAP2A/AP2α) at neural stem cell (NSC) stage and across different passages of NCR cell culture (NCR P0 to P9)