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. 2022 Jun 21;18(8):2952–2965. doi: 10.1007/s12015-022-10393-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Lineage marker analysis confirms the presence of distinct developmental stages in long-term neural crest culture. (a) Immunocytochemical analysis of different passages throughout NCR differentiation (P0, P3, P6 and P9) for neural progenitors (DCX and SOX2), NCR (HNK1/CD57) and mesenchymal cells (CD44, Vimentin) confirms the presence of clearly distinct phenotypic stages in long-term NCR culture in congruence with morphological changes (Scale bar: 50 μm)