Table 2.

Comparison of microglia staining intensity and cell numbers.

Group comparisons	(A) Iba-1 intensity	(B) Number of cells
	Ipsilateral	Ipsilateral
RSN vs. CSN (stress)
p Value	p = 0.9908	p = 0.7075
Cohen d (effect size)	0.2	0.7 ↑
CNN vs. CSN (1st injection)
p Value	p = 0.8515	p = 0.9438
Cohen d (effect size)	0.7 ↑	0.7 ↑
RSN vs. RSS (2nd injection)
p Value	p = 0.9984	p = 0.6539
Cohen d (effect size)	0.0	0.6 ↑

The table shows the statistics for (A) Iba-1 staining intensity and (B) number of microglia cells, on the ipsilateral side of NGF/saline injections. The staining intensity and number of cells were analyzed for each ROI and averaged across the four ipsilateral ROIs within each animal. The statistics were performed using the mean ± SEM (n = 5 animals per group). P < 0.05 (two-way ANOVA followed by Tukey post hoc analysis) was considered significant and effect size was calculated using Cohen d. The group comparisons shown here are based on two factors: 1) repeated restraint stress (R) and control (C), 2) NGF (N) and saline (S) injections. Iba-1, ionized calcium binding adapter molecule 1; NGF, nerve growth factor. CSN: control + saline + NGF; RSS: repeated restraint stress + saline + saline; CNN: control + NGF + NGF; and RSN: repeated restraint stress + saline + NGF.