Table 3.

Comparison of microglia cell numbers in different states.

Group comparisons	A		B
	Ipsilateral		SDH		DDH
Number of cells	p Value	Cohen d	p Value	Cohen d	p Value	Cohen d
RSN vs. CSN (Stress)
Surveilling state	p = 0.9260	0.4	p = 0.8524	0.6 ↑	p = 0.9923	0.2
Hyper-ramified state	p = 0.7863	0.6 ↑	p = 0.9164	0.4	p = 0.7719	0.6 ↑
Bushy state	p = 0.9757	0.3	p = 0.7713	0.7 ↑	p = 0.9725	0.2
Ameboid state	p = 0.1045	1.2 ↑	p = 0.1295	1.1 ↑	p = 0.3932	0.9 ↑
CNN vs. CSN (1st injection)
Surveilling state	p = 0.0359*	1.8 ↑	p = 0.1017	1.3 ↑	p = 0.2277	1.3 ↑
Hyper-ramified state	p = 0.9994	0.1	p = 0.6882	0.6	p = 0.9825	0.2
Bushy state	p = 0.9998	0.0	p = 0.9865	0.3	p = 0.9193	0.5 ↓
Ameboid state	p = 0.4323	0.7 ↓	p = 0.5006	0.8 ↓	p = 0.5253	1.2 ↓
RSN vs. RSS (2nd injection)
Surveilling state	p = 0.5247	0.9 ↓	p = 0.5469	1.0 ↓	p = 0.7421	0.7 ↓
Hyper-ramified state	p = 0.9980	0.1	p = 0.9651	0.4	p = 0.9997	0.0
Bushy state	p = 0.9711	0.2	p = 0.9744	0.2	p = 0.9725	0.3
Ameboid state	p = 0.0044**	3.7 ↑	p = 0.0032**	3.4 ↑	p = 0.1942	1.0 ↑

The table shows the statistics for number of microglia cells in different morphological states (A) on the ipsilateral side and (B) on the superficial and deep dorsal horn on the ipsilateral side. The number of cells were counted for each ROI and averaged across ROIs within each animal. The statistics were performed using the mean ± SEM. P < 0.05 (two-way ANOVA followed by Tukey post hoc analysis) was considered significant indicated by * and effect size was calculated using Cohen d. The group comparisons shown here are based on the factors: 1) repeated restraint stress (R) and control (C), 2) NGF (N) and saline (S) injections. ROI, region of interest; SDH, superficial dorsal horn; DDH, deep dorsal horn; Iba-1, ionized calcium binding adapter molecule 1; NGF, nerve growth factor. CSN: control + saline + NGF; RSS: repeated restraint stress + saline + saline; CNN: control + NGF + NGF; and RSN: repeated restraint stress + saline + NGF.