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. 2022 Nov 1;8:118. doi: 10.1038/s41421-022-00474-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a RNAscope analysis of hepatic expression of Gli1 (red) and EpCAM (green) or CK19 (green) on 8-week-old WT mice. Scale bars, 200 μm. b Experimental design for lineage tracing of Gli1⁺ cells using Gli1-Cre^ERt2;Ai9 mice. c Immunostaining for tdTomato (red) and EpCAM (green), PDGFRα (green) or α-SMA (green) on liver sections (left panels). White arrowheads indicate the co-stained cells. Scale bars, 50 μm. Quantification of the percentage of tdTomato⁺ cells expressing PDGFRα, EpCAM, and α-SMA (right panels). Data are presented as means ± SEM (n = 3). d Immunostaining for tdTomato (red), EpCAM (white), and PDGFRα (green) or α-SMA (green) in liver sections. PV portal vein. White arrowhead indicates the co-stained cell. Scale bars, 50 μm.