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. 2022 May 16;43(11):2929–2945. doi: 10.1038/s41401-022-00914-6

Fig. 4. AhR signalling was activated by MP in kidney proximal tubular epithelial cells, macrophages and kidney interstitial fibroblasts.

Fig. 4

a The viability of NRK-52E cells treated with increasing concentrations of MP (0–400 nM) for 24 h and 10 nM MP for different times (0–48 h). b AhR mRNA expression in NRK-52E cells after treatment with increasing concentrations of MP for 24 h and 10 nM MP for different times. c Protein expression of AhR in the nucleus and cytoplasm of NRK-52E cells induced by different concentrations of MP. d Quantitative analysis of AhR expression in NRK-52E cells induced by different concentrations of MP. e Protein expression of AhR in the nucleus and cytoplasm of NRK-52E cells induced by MP for different times. f Quantitative analysis of AhR expression in NRK-52E cells induced by MP for different times. g AhR mRNA expression in RAW 264.7 cells after treatment with increasing concentrations of MP for 24 h and 10 nM MP for different times. h Protein expression of AhR in the nucleus and cytoplasm of RAW 264.7 cells induced by different concentrations of MP. i Quantitative analysis of AhR expression in RAW 264.7 cells induced by different concentrations of MP. j Protein expression of AhR in the nucleus and cytoplasm of RAW 264.7 cells induced by MP for different times. k Quantitative analysis of AhR expression in RAW 264.7 cells induced by MP for different times. l AhR mRNA expression in NRK-49F cells after treatment with increasing concentrations of MP for 24 h and 10 nM MP for different times. m Protein expression of AhR in the nucleus and cytoplasm of NRK-49F cells induced by different concentrations of MP. n Quantitative analysis of AhR expression in NRK-49F cells induced by different concentrations of MP. o Protein expression of AhR in the nucleus and cytoplasm of NRK-49F cells induced by MP for different times. p Quantitative analysis of AhR expression in NRK-49F cells induced by MP for different times. q Luciferase assay of AhR activation in MP-induced NRK-52E cells. r Luciferase assay of AhR activation in MP-induced RAW 264.7 cells. s Luciferase assay of AhR activation in HP-induced NRK-49F cells. t The mRNA expression levels of AhR target genes, including CYP1A1, CYP1A2, CYP1B1 and COX-2, in MP-induced NRK-52E cells, RAW 264.7 cells and NRK-49F cells. *P < 0.05, **P < 0.01 vs the CTL group.