Fig. 5.
The role played by S1PR1 on donor T cells in GVH and GVL responses. Lethally irradiated BALB/c mice (700 cGy) underwent BMT with 5 × 106 TCD-BMCs alone or with 0.75 × 106 total T cells isolated from WT or S1PR1flox/floxCD4-Cre mice, and recipients were monitored for A body weight loss, B clinical score and C survival over time (n = 10 mice/group). Tissues from BALB/c recipients were collected on 21 days after allo-BMT and analyzed for pathology. D Representative photomicrographs showing hematoxylin and eosin staining of livers, small and large intestines at the original magnification 200x, and E pathology scores are shown (n = 6–10 mice/group). Lethally irradiated B6D2F1 recipients were transplanted with 5 × 106 TCD-BMCs alone or with 3 × 106 total CD25-depleted T cells isolated from WT or S1PR1flox/floxCD4-Cre mice and with or without 5000 β-actin-luciferase-transduced P815 mastocytoma cells. Mice were monitored for tumor burden. F BLI images taken throughout the experiment were used to determine tumor growth. G Body weight loss, H clinical score, I survival, and J tumor mortality of recipients were monitored over time (n = 10 mice/group). The experiments were repeated 2 to 3 independent times, and the combined data are presented. Log-rank (Mantel‒Cox) test (C, I, and J) and nonparametric Mann‒Whitney U test (A, B, G and H) were performed to compare between groups. Statistical data are presented as the mean ± 1 SD, and significance was determined by Student’s t-test. *P < 0.05, **P < 0.01 and ***P < 0.001