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. 2022 May 17;43(11):2977–2992. doi: 10.1038/s41401-022-00917-3

Fig. 4. Knockdown of APOC1 promoted ferroptosis by reduced expression of HO-1 and NQO1 expression.

Fig. 4

a Overexpression of APOC1 attenuated ROS level which can be reversed by knockdown of HO-1 and NQO1 expression in U251 cells. b mRNA expression of HO-1 and NQO1 was increased in U87 cells after treatment of Erastin for 24 h. c Knockdown of APOC1 expression decreased HO-1 and NQO1 mRNA level in U87 cells treated by Erastin for 24 h. d Knockdown of HO-1 or NQO1 expression increased ROS level of U87 cells treated by Erastin for 24 h. e Knockdown of HO-1 or NQO1 increased intracellular Fe2+ of U251 cells in Erastin-induced ferroptosis. Representative images of Fe2+ with identical results in three assays were shown. Scale bar = 50 μm. f Knockdown of HO-1 or NQO1 increased lipid ROS level of U87 cells. Experiments were performed in triplicate. Data are presented as mean ± SD (n = 3). Statistical significance was determined by Student’s t test or one-way ANOVA, **P < 0.01, ***P < 0.001 vs. NC.