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. 2022 Oct 31;8:436. doi: 10.1038/s41420-022-01226-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A FRAP of NOP53-mEGFP puncta in the nucleoplasm of HEK293T cells. B The NoLS of NOP53 was analyzed with NOD (www.compbio.dundee.ac.uk/software.html#3Dstructure) and verified in HEK293T cells. C Schematic of R motifs or M-R motifs distribution in NOP53 sequence and truncation of NOP53-IDR1/IDR2 sequence. R motifs were defined as the sequence pattern, RX_n1R (n1 ≤ 2), while M-R motifs were defined as the sequence pattern, RX_n1RX_n2RX_n3R (n1 ≤ 2, n3 ≤ 2, and n2 ≤ 20). D Distribution of NOP53-Rm-mEGFP, NOP53-Rm+NoLS-mEGFP and NOP53-Rm-del(41-159)-mEGFP in HEK293T cells. E The purified NOP53-Rm-mEGFP and NOP53-Rm+NoLS-mEGFP protein, but not NOP53-Rm-del(41-159)-mEGFP protein, formed droplets in vitro. One micromolar protein was used.