Extended Data Figure 3. The cBAF complex but not the PBAF complex affects CD8⁺ T cell differentiation.

a, Immunoblot of Pbrm1 in naïve CD8⁺ T cells from Pbrm1^fl/fl and Cd4^CrePbrm1^fl/fl mice. b−e Pbrm1^fl/fl and Cd4^CrePbrm1^fl/fl mice were challenged with Lm-Ova and analyzed at day 7.5 (b, c) or at day >30 post-infection (d, e). b, Flow cytometry analysis (left) and quantification (right) of the frequencies and numbers of total CD8⁺ T cells (upper) and Ova-specific CD8⁺ T cells (lower) (Pbrm1^fl/fl, n=8 mice; Cd4^CrePbrm1^fl/fl, n=6 mice). c, Flow cytometry analysis (left) and quantification (right) of Ova-specific TE (KLRG1^hiCD127^lo), MP (KLRG1^loCD127^hi), CXCR1^hiKLRG1^hi and CD62L⁺ cells in spleen (n=6 per group). d, Flow cytometry analysis (left) and quantification (right) of Ova-specific T_MEM cells in the spleen and indicated organs (Pbrm1^fl/fl, n=5 mice; Cd4^CrePbrm1^fl/fl, n=8 mice). e, Percentage of splenic CD62L⁺ T_CM population (Pbrm1^fl/fl, n=5 mice; Cd4^CrePbrm1^fl/fl, n=8 mice). f, Flow cytometry analysis of splenic TE (KLRG1^hiCD127^lo), MP (KLRG1^loCD127^hi) and CD62L⁺ OT-I cells transduced with sgNTC or the indicated sgRNA targeting ncBAF complex components at day 7.5 post Lm-Ova infection (Pbrm1^fl/fl, n=5 mice; Cd4^CrePbrm1^fl/fl, n=8 mice). g, Flow cytometry analysis of splenic TE (KLRG1^hiCD127^lo), MP (KLRG1^loCD127^hi) and CD62L⁺ OT-I cells transduced with sgNTC or the indicated sgRNA at day 7.5 post Lm-Ova infection. Data are compiled from two (b−e) or representative of two (a, f, g) independent experiments. Data are shown as mean±s.e.m. **p<0.01, ns, not significant; two-tailed unpaired Student’s t-test (b−g).