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. 2022 Oct 18;12:1004793. doi: 10.3389/fonc.2022.1004793

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Copyright © 2022 Rada, Hassan, Lazaris and Metrakos

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RUNX1 knockdown in the cancer cells attenuates neutrophil infiltration. (A) Represents immunohistochemical staining of CRCLM sections generated from HT29 cancer cells expressing shRNA-Scrambled (n=5) or shRNA-RUNX1 (n=5). We used anti-RUNX1 and anti-LY6G (neutrophil biomarker) antibodies. (B) Using Pearson correlation analysis, we showed the correlation between RUNX1 expression and LY6G expression in CRCLM sections. Relative expression levels of RUNX1 or LY6G were calculated by dividing the positivity value of each specimen from shRNA-Scrambled and shRNA-RUNX1 groups by the positivity value of specimen #1 from shRNA-Scrambled group and then presenting the result as a fold difference.