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The parameter of Dionex ultimate 3,000 nano LC system.
| Item | Parameter |
|---|---|
| Nano LC trap | Acclaim pepmap 100; 150 μm × 2 cm nanoviper C18 5 μm 100A |
| Nano LC column | C18 5 μm 75 μm × 15 μm 300A |
| Solvent A | 0.1% formic acid 2% ACN 98% water |
| Solvent B | 0.1% formic acid 2% water 98% ACN |
| Flow rate | 0.4 μL/min |
