Table 3.
The parameter of identification and retrieval.
| Parameter name | Parameter | Experimental options |
|---|---|---|
| Mascot version number | Mascot version | 2.3.0 |
| Fixed modification | Fixed modification | Carbamidomethyl (C), itraq 8 plex (K), itraq 8 plex (N-term) |
| Variable modification | Variable modification | Oxidation (M), Gln → Pyro-Glu (N-term Q), Itraq 8 plex (Y) |
| Primary quality deviation | Peptide tol | 15 ppm |
| Secondary quality deviation | MS/MS tol | 20 mmu |
| Maximum allowable missed cleavage | Max missed cleavages | 1 |
| Enzyme typ | Enzyme | Trypsin |
| Database | Database | 2019-uni-human; Time files compressed: 2019.10.22; Number of sequences: 172097 |