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. 2022 Oct 18;16:1032195. doi: 10.3389/fnins.2022.1032195

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Copyright © 2022 Wang, Qiao, Jiang, Chen, Lu, Ning, Jin, Gong and Yuan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Imaging principle of NM-ILSM. (A) Schematic diagram of the system configuration. (B) ILSM mode: imaging the sample S with FOV_O. NM-ILSM mode: imaging the sample S_n again after ILSM mode with FOV_E. CL, cylindrical lens; L, lens; EO, excitation objective; DO, detection objective; TL, tube lens; sCMOS, scientific complementary metal–oxide–semiconductor detector. (C,D) Data acquisition flow of large-volume 3D imaging by the updated system combined with a customized vibratome in ILSM and NM-ILSM modes, respectively. (E) A typical example showing maximum intensity projections (MIP) with a 20-μm-thickness in the central and extended FOVs, and their NM-ILSM reconstructed image.