Table 1.
Characteristics of different expression genes related to immune function.
| Target gene | Primer sequence Forward (5’→3’) | Primer sequence Reverse (5’→3’) | Temperature(°C) | Accession number |
|---|---|---|---|---|
| hepcidin | AGCAGGAGCAGGATGAGC | GCCAGGGGATTTGTTTGT | 59.3 | JQ246442.1 |
| LEAP-2A | TGCCTACTGCCAGAACCA | AATCGGTTGGCTGTAGGA | 59.3 | FJ390414 |
| LEAP-2B | TGTGCCATTAGCGACTTCTGAG | ATGATTCGCCACAAAGGGG | 59.3 | KT625603 |
| β-defensin-1 | TTGCTTGTCCTTGCCGTCT | AATCCTTTGCCACAGCCTAA | 58.4 | KT445868 |
| Mucin2 | GAGTTCCCAACCCAACACAT | AAAGGTCTACACAATCTGCCC | 60.4 | KT625602 |
| IFN-γ2 | TGTTTGATGACTTTGGGATG | TCAGGACCCGCAGGAAGAC | 60.4 | JX657682 |
| TNF-α | CGCTGCTGTCTGCTTCAC | CCTGGTCCTGGTTCACTC | 58.4 | HQ696609 |
| IL-1β | AGAGTTTGGTGAAGAAGAGG | TTATTGTGGTTACGCTGGA | 57.1 | JQ692172 |
| IL-6 | CAGCAGAATGGGGGAGTTATC | CTCGCAGAGTCTTGACATCCTT | 62.3 | KC535507.1 |
| IL-8 | ATGAGTCTTAGAGGTCTGGGT | ACAGTGAGGGCTAGGAGGG | 60.3 | JN663841 |
| IL-10 | AATCCCTTTGATTTTGCC | GTGCCTTATCCTACAGTATGTG | 61.4 | HQ388294 |
| IL-12p35 | TGGAAAAGGAGGGGAAGATG | AGACGGACGCTGTGTGAGTGTA | 55.4 | KF944667.1 |
| IL-12p40 | ACAAAGATGAAAAACTGGAGGC | GTGTGTGGTTTAGGTAGGAGCC | 59.0 | KF944668.1 |
| IL-15 | CCTTCCAACAATCTCGCTTC | AACACATCTTCCAGTTCTCCTT | 61.4 | KT445872 |
| IL-17D | GTGTCCAGGAGAGCACCAAG | GCGAGAGGCTGAGGAAGTTT | 62.3 | KF245426.1 |
| IL-4/13A | CTACTGCTCGCTTTCGCTGT | CCCAGTTTTCAGTTCTCTCAGG | 55.9 | KT445871 |
| IL-4/13B | TGTGAACCAGACCCTACATAACC | TTCAGGACCTTTGCTGCTTG | 55.9 | KT625600 |
| TGF-β1 | TTGGGACTTGTGCTCTAT | AGTTCTGCTGGGATGTTT | 55.9 | EU099588 |
| TGF-β2 | TACATTGACAGCAAGGTGGTG | TCTTGTTGGGGATGATGTAGTT | 55.9 | KM279716 |
| NF-κB p52 | TCAGTGTAACGACAACGGGAT | ATACTTCAGCCACACCTCTCTTAG | 58.4 | KM279720 |
| NF-κB p65 | GAAGAAGGATGTGGGAGATG | TGTTGTCGTAGATGGGCTGAG | 62.3 | KJ526214 |
| c-Rel | GCGTCTATGCTTCCAGATTTACC | ACTGCCACTGTTCTTGTTCACC | 59.3 | KT445865 |
| IκBα | TCTTGCCATTATTCACGAGG | TGTTACCACAGTCATCCACCA | 62.3 | KJ125069 |
| IKKα | GGCTACGCCAAAGACCTG | CGGACCTCGCCATTCATA | 60.3 | KM279718 |
| IKKβ | GTGGCGGTGGATTATTGG | GCACGGGTTGCCAGTTTG | 60.3 | KP125491 |
| IKKγ | AGAGGCTCGTCATAGTGG | CTGTGATTGGCTTGCTTT | 58.4 | KM079079 |
| TOR | TCCCACTTTCCACCAACT | ACACCTCCACCTTCTCCA | 61.4 | JX854449 |
| S6K1 | TGGAGGAGGTAATGGACG | ACATAAAGCAGCCTGACG | 54.0 | EF373673 |
| 4E-BP1 | GCTGGCTGAGTTTGTGGTTG | CGAGTCGTGCTAAAAAGGGTC | 60.3 | KT757305 |
| 4E-BP2 | TGTGCCATTAGCGACTTCTGAG | ATGATTCGCCACAAAGGGG | 59.3 | KT625603 |
| β-actin | GGCTGTGCTGTCCCTGTA | GGGCATAACCCTCGTAGAT | 61.4 | M25013 |