Table 4.
Carotenoid content and composition (µg g-1 DW), and ABA, ABA-GE and ABA catabolites (Fold Internal Standard) levels in bark and wood of young stems of Valencia late (A) and Ruby Valencia mutant (B) orange trees.
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|
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|---|---|---|---|---|
| Carotenoids (µg g-1 DW) | Valencia | Ruby | ||
| Bark | Wood | Bark | Wood | |
| Phytoene | 5.45 ± 1.81* | 1.16 ± 0.05* | 389.45 ± 20.58 | 139.01 ± 32.51 |
| Phytofluene | nd | nd | 38.35 ± 1.38 | 5.10 ± 3.31 |
| α-carotene | 2.01 ± 1.00 | nd | 4.27 ± 1.00 | nd |
| Lutein | 104.99 ± 4.14* | 1.09 ± 0.11 | 56.57 ± 3.78 | nd |
| β-carotene | 4.93 ± 1.20 | nd | 4.30 ± 0.64 | nd |
| Lycopene | nd | nd | 0.65 ± 0.08 | traces |
| Antheraxanthin | 4.72 ± 2.67 | nd | nd | nd |
| Violaxanthin | 31.56 ± 1.56* | 0.10 ± 0.10 | 11.46 ± 0.20 | nd |
| Neoxanthin | 23.16 ± 0.86* | nd | 11.66 ± 3.81 | nd |
| Total carotenoids | 176.82 ± 8.63* | 2.35 ± 0.04* | 516.70 ± 28.29 | 146.29 ± 35.76 |
| ABA and ABA-related metabolites(Fold IS) | ||||
| ABA | 0.17 ± 0.04* | 0.16 ± 0.01* | 0.03 ± 0.01 | 0.03 ± 0.01 |
| ABA-GE | nd | nd | 0.04 ± 0.01 | nd |
| DA | 19.60 ± 1.54* | 0.16 ± 0.02 | 4.83 ± 0.26 | 0.15 ± 0.01 |
| PA | 1.80 ± 0.14* | 0.02 ± 0.01 | 0.16 ± 0.01 | 0.03 ± 0.01 |
The amount of violaxanthin corresponds to the sum of all-E-violaxanthin, all-Z-violaxanthin isomers and luteoxanthin. ABA, abscisic acid; ABA-GE, abscisic acid-glucose ester; DA, dihydrophaseic acid; PA, phaseic acid. Nd, no detected. Pictures represent the tissues used for carotenoid and ABA analyses. Data are the mean of three biological replicates ± SD. Asterisks indicate significant differences between Valencia and Ruby varieties by unpaired Student’s t-test (p<0.05).