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. 2022 Oct 31;220(1):e20220837. doi: 10.1084/jem.20220837

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© 2022 Jenster et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S5. — Supplementary immunoblots and SDS-PAGE gels for Figs. 9 and 10. (A and C) Lysates of HEK 293T (HEK) or HEK^ASC-based cell lines constitutively expressing HA-tagged human NLRP1, the indicated murine Nlrp1b alleles (A), or the indicated human NLRP1 mutants (A and C) were analyzed by immunoblot with the indicated antibodies to verify expression of NLRP1-HA variants and ASC-EGFP. (B) Purified MBP-NLRP1 and MBP-NLRP1^NACHT+LRR (left), as well as purified, activated p38α-His (right) used in the in vitro kinase assay in Fig. 9 B were analyzed by SDS-PAGE and Coomassie staining. (D) Lysates of N/TERT-1^C1C-EGFP, monoclonal N/TERT-1^C1C-EGFP ΔNLRP1, or N/TERT-1^C1C-EGFP ΔNLRP1 reconstituted with the dox-inducible expressed NLRP1 or the indicated NLRP1 mutants were analyzed by immunoblot with the indicated antibodies to verify constitutive expression of C1C-EGFP and dox-induced expression of NLRP1 after 6 h. Data displays experiment representatives of two independent experiments. MW, molecular weight. Source data are available for this figure: SourceData FS5.