Table 5.

Comparison of pAtGCSpro ₁₁₇₈-Cas9-SlTRY and p2×35Spro-Cas9-SlTRY genome editing efficiency in tomato hairy roots.

Cas9 system	H/BM rate at target site 1 (no. of roots with H/BM at target site 1/no. of roots examined)	H/BM rate at target site 2 (no. of roots with H/BM at target site 2/no. of roots examined)	H/BM rate at both target sites 1 and 2 (no. of roots with H/BM at both target site 1 and 2/no. of roots examined)
p2×35Spro-Cas9- SlTRY	23.3% (7/30)	13.3% (4/30)	6.7% (2/30)
pAtGCSpro 1178-Cas9-SlTRY	91.3% (21/23)	78.3% (18/23)	65.2% (15/23)