KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-CREB3L2 C terminus | Abcam | ab102989; RRID:AB_10711653 |
| Rabbit polyclonal anti-CREB3L2 C terminus | Abcepta | AP9654b; RRID:AB_2544158 |
| Rabbit polyclonal anti-CREB3L2 N terminus | MilliporeSigma | HPA015068; RRID:AB_1847224 |
| Mouse monoclonal Alexa Fluor 488 anti-βIII-Tubulin (TUJ1 clone) | BioLegend | 801203; RRID:AB_2564757 |
| Mouse monoclonal anti-SHH (E-1) | Santa Cruz | sc-365112; RRID:AB_10709580 |
| Rabbit polyclonal anti-PTCH1 | Proteintech | 17520-1-AP; RRID:AB_2176561 |
| Mouse monoclonal anti-SMO (E-5) | Santa Cruz | sc-166685; RRID:AB_2239686 |
| Rabbit polyclonal anti-GLI1 (V812) | Cell Signaling Technology | 2534; RRID:AB_2294745 |
| Rabbit polyclonal anti- GFP | Abcam | ab290; RRID:AB_303395 |
| Rabbit polyclonal anti-cleaved CASP3 | Cell Signaling Technology | 9661; RRID:AB_2341188 |
| Rabbit polyclonal anti-PARP | Cell Signaling Technology | 9542; RRID:AB_2160739 |
| Mouse monoclonal anti-β-Actin (AC-15) | Abcam | ab6276; RRID:AB_2223210 |
| Mouse monoclonal anti-GAPDH (6C5) | Santa Cruz | sc-32233; RRID:AB_627679 |
| Rabbit monoclonal anti-p-eIF2α | Cell Signaling Technology | 3398; RRID:AB_2096481 |
| Rabbit monoclonal anti-ATF4 | Cell Signaling Technology | 11815; RRID:AB_2616025 |
| Rabbit monoclonal anti-NRF2 (D1Z9C) | Cell Signaling Technology | 12721; RRID:AB_2715528 |
| Mouse monoclonal anti-KEAP1 | Proteintech | 60027-1-lg; RRID:AB_2132623 |
| Mouse monoclonal anti-TOM20 (2F8.1) | MilliporeSigma | MABT166; RRID:AB_2890986 |
| Bacterial and virus strains | ||
| Stellar competent cells | Takara | 636766 |
| Biological samples | ||
| See Table S1. A complete list of all human brain samples used in this study | New York Brain Bank | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| DMSO | MilliporeSigma | D8418 |
| Nelfinavir | MilliporeSigma | CDS021783 |
| Robotnikinin | Thermo Fisher Scientific | 50464659 |
| 4-Hydroxynonenal (HNE) | MilliporeSigma | 3932041MG |
| Purmorphamine | MilliporeSigma | 5402235MG |
| MG132 | Cayman Chemical | 133407-82-6 |
| SIN-1 | MilliporeSigma | 567028 |
| Hydrogen peroxide | MilliporeSigma | MAK092C |
| Chloroquine diphosphate | Tocris Bioscience | 4109/50 |
| Tunicamycin | MilliporeSigma | 50-457-00001 |
| ΔBD (recombinant protein) | His-tag purification | N/A |
| TAILS (recombinant protein) | His-tag purification | N/A |
| Critical commercial assays | ||
| Luna Universal Probe One-Step RT-qPCR Kit | NEB | E3006L |
| Duolink In Situ Detection Reagents (Red) | MilliporeSigma | DUO92008 |
| Duolink In Situ Detection Reagents (FarRed) | MilliporeSigma | DUO92013 |
| Duolink In Situ PLA Probe anti-Mouse PLUS | MilliporeSigma | DUO92001 |
| Duolink In Situ PLA Probe anti-Rabbit MINUS | MilliporeSigma | DUO92005 |
| In Situ Cell Death Detection Kit (TMR red) | Roche | 12156792910 |
| Peroxidase Activity Assay Kit | MilliporeSigma | MAK092 |
| CellROX (Green) | Invitrogen | C10444 |
| AlamarBlue Cell Viability Assay | G-Biosciences | 786922 |
| GSH/GSSG-Glo Glutathione Assay | Promega | V6611 |
| Seahorse XFp FluxPak | Agilent Technologies | 103022-100 |
| Seahorse XFp Cell Mito Stress Test Kit | Agilent Technologies | 103010-100 |
| Capturem His-Tagged Purification Kit | Takara | 635710 |
| CyQUANT Cell Proliferation Assay | Thermo Fisher Scientific | C7026 |
| CalFectin Transfection Reagent | SignaGen Laboratories | SL100478 |
| Deposited data | ||
| Single-nucleus RNA-sequencing database | Yang et al., 2022 | GSE163577; https://twc-stanford.shinyapps.io/human_bbb |
| Experimental models: Cell lines | ||
| Human: HEK293T | ATCC | CRL-3216 |
| Mouse: Neuro2a | ATCC | CCL-131 |
| Human: HEK293T (constitutive TAILS secretion) | This paper | N/A |
| Experimental models: Organisms/strains | ||
| Rat: Hsd:Sprague Dawley SD (pregnant) | Envigo | N/A |
| Rat: Hsd:Sprague Dawley SD (adult male) | Charles River Laboratories | N/A |
| Oligonucleotides | ||
| Gss (Rn00564188_m1) | Thermo Fisher Scientific | 4331182 |
| Nfe2l2 (Rn00821258_g1) | Thermo Fisher Scientific | 4331182 |
| Rpl13 (Rn00582415_m1) | Thermo Fisher Scientific | 4331182 |
| Gss (Rn00564188_m1) | Thermo Fisher Scientific | 4331182 |
| Recombinant DNA | ||
| 7xGLI-GFP | Addgene | 110494 |
| 8xARE-GFP | Addgene | 134910 |
| pCMV-erHRP (N175S mutant) | Addgene | 79909 |
| C-terminal CREB3L2 (His-tagged) | McCurdy et al., 2019 | N/A |
| TAILS (BiP sequence, His-tagged) | This paper | N/A |
| ΔBD (binding domain deletion mutant; BiP sequence, His-tagged) | This paper | N/A |
| CREB3L2 (HRP-tagged) | This paper | N/A |
| Software and algorithms | ||
| ImageJ (FIJI) | Schindelin et al., 2012 | RRID:SCR_002285 |
| ANY-Maze | Stoelting | RRID:SCR_014289 |
| GraphPad Prism 9.4 | GraphPad Software | RRID:SCR_002798 |