Cell viabilities of (a) MCF-7, (b) PANC-1,
and (c) HepG2 cells
treated with MOF-808 and its drug loaded and PAAMAM coated formulations,
compared to cell viabilities of (d) MCF-7, (e) PANC-1, and (f) HepG2
cells treated with MOF-808_act and its drug loaded and PAAMAM coated
formulations. Cell viabilities of the three cell lines when treated
with (g) free FUDR, (h) free CARB, and (i) PAAMAM are also provided
for comparison. All incubations carried out for 72 h. Viabilities
assessed by the Alamar blue cell viability assay with untreated cells
accepted as control. The data were expressed as mean and standard
deviation (n = 3 × 3), p ≤
0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***), and p ≤ 0.0001 (****).
The stars show the statistical significance of each data point from
the control. An inset for part g to show statistical significance
below 10 μg mL–1 is provided in Figure S38. The equivalent FUDR and CARB concentrations
for each nanoparticle concentration used (between 5 and 100 μg
mL–1) are reported in Table S1, and the data plotted as a function of CARB concentration
are given in Figure 5, where appropriate.