Table 1.
Different small extracellular vesicle purification and isolation methods applied in bovine colostrum
| Sample1 | Purification kit | RNA isolation kit | RNA concentration2 (ng/μL) | miRNA library quality check3 |
|---|---|---|---|---|
| 1 | Plasma/Serum Exosome Purification Kit (Norgen Biotek Corp.) | Exosome RNA Isolation Kit (Norgen Biotek Corp.) | 18.05 | Passed |
| 2 | miRCURY Exosome Cell/Urine/CSF Kit (Qiagen) | Exosome RNA Isolation Kit (Norgen Biotek Corp.) | Failed | Failed |
| 3 | miRCURY Exosome Cell/Urine/CSF Kit (Qiagen) | miRNeasy Mini Kit (Qiagen) | 45.46 | Passed |
1
Three colostrum samples were collected from 3 dairy cows at the Dairy Research and Technology Centre (University of Alberta, Edmonton, Canada). Each colostrum sample was subjected to one combination of purification and RNA isolation kits.
2
The RNA concentration was measured using a Qubit 3.0 Fluorometer (Invitrogen) using 1 μL of RNA (1–1.5 mL of samples).
3
Quality check was done by analyzing 1 μL of the miRNA Sequencing Library on a 2200 TapeStation System (Agilent Technologies) using a High Sensitivity DNA chip according to the manufacturer's instructions.