Table 3.

Antiviral activity of compound 3 against some SARS-CoV-2 mutants

Strains	5h (2)	3
	EC50 (μM)	EC50 (μM)
WK-521 (ancestral Wuhan strain)	2.6 ± 0.070	0.28 ± 0.02
QHN001 (α)	2.3 ± 0.090	0.34 ± 0.09
TY8-612 (β)	2.2 ± 0.050	0.22 ± 0.035
TY7-501 (γ)	2.4 ± 0.050	0.28 ± 0.060
K1734 (δ), Exp. 1	2.7 ± 0.075	0.35 ± 0.15
K1734 (δ), Exp. 2	not tested	0.59 ± 0.24
K5356 (κ)	2.4 ± 0.13	0.29 ± 0.065
00012 (ο, ΒΑ.1)	Not tested	0.47 ± 0.050
02037 (ο, ΒΑ.2)	Not tested	0.33 ± 0.10

EC₅₀ values were determined with RNA-qPCR and CC₅₀ values with WST-8 assays, using VeroE6 cells.

Numbers represent the average EC₅₀ value ± SD (μM) and CC₅₀ value ± SD (μM) from two independent experiments.

Exp.: experiment.