Fig. 6.

CD4 + CD28− T cells induce higher apoptosis of GEnCs with IL-15 pre-treatment compared with CD4 + CD28 + T cells

After 16 h stimulation of 10⁵ CD4 + CD28 + or CD4 + CD28− T cells isolated from LN patients (n = 7) with IL-15 50ng/ml and 6h culture with GEnCs, the frequency of GEnCs expressing active caspase 3 and intracellular IFN-γ production in T cells were measured by flow cytometry

(A) Expression of active caspase 3 of GEnCs (upper left), induced by CD4 + CD28 + T cells (bottom left), CD4 + CD28− T cells (upper right), the CD4 + CD28− T cells with anti-NKG2D blocking antibody (bottom right)

(B) Data show the mean ± SEM normalized apoptosis rate of GEnCs in each condition

(C) Data show the mean ± SEM normalized apoptosis rate of GEnCs without IL-15 treatment and with IL-15 treatment CD4 + CD28− T cells

(D) Data show the mean ± SEM IFN -γ production of CD4 + CD28 + or CD4 + CD28− T cells in each condition. *p < 0.05, **p < 0.01, ns means no significance