Figure 5.

Loading-induced periosteal bone formation is maintained in mice with mature osteoblast lineage deletion of VEGFA using Dmp1 Cre. A) Representative images from control and four loaded groups demonstrate increased periosteal label around the entire tibia after loading (scale bar 500 micron). Magnified images of the area of peak compression demonstrate increased label and spacing between labels on loaded bones (scale bar 50 micron). B) Indices of periosteal bone formation in female control (Vegfa^fl/fl) and conditional knockout (VegfaΔ^Dmp1) mice (n = 5–9). C) Indices of periosteal bone formation in male control (Vegfa^fl/fl) and conditional knockout (VegfaΔ^Dmp1) mice (n = 4–7). Two-factor ANOVA was used to evaluate the effects of limb (loading) and genotype, with Tukey post hoc comparisons. One-way ANOVA was used to compare relative bone formation rate (BFR/BS Loaded minus BFR/BS Non-loaded), which was used as an index of loading-induced bone formation. Bars depict mean ± SD, with individual data points shown. Parameters shown do not include woven bone. * p<0.05